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    ˮ۱ϩPAM

    ³֮Դ ʱ䣺20-09-28

    ˮ۱ϩPAMоӦ״뷢չ 

        ˮĸ߷ʡˮ۱ϩPAMȻɽ⣬ȫ޶޶ȾЩܵо߼עˮ۱ϩPAM\ˮȡõһְȫЧȻˮˮ۱ϩPAMԿ˷޻߷Ӻͺϳл߷Ӿ۱ϩPAMеȱݣʵȾŷţˮ۱ϩPAMоΪ۱ϩPAMоҪ⡣ˮ۱ϩPAMƺӦ÷δԺΪˮķչչʾһǰˮ۱ϩPAMδȡ򲿷ȡͳ޻߷Ӻͺϳл߷Ӿ۱ϩPAMҹˮ۱ϩPAMо𲽽λȽϵͣ󻷾ͶзչΪҹ۱ϩPAMķչ춨ۺʻ 
        1ˮ۱ϩPAMķչ 
        1.1ˮ۱ϩPAMо 
        ˮ()ˮʹõľ۱ϩPAMҪࣺϵϵΪ޻߷Ծ۱ϩΪĺϳл߷Ӿ۱ϩPAMйоˮӵȺУԳմ֢Ļ߱ϸߡȫ˿仯ijֺͼӾ磬ǶϵʹðȫɡϩǾǿҵ񾭶Ժ°á
        ⣬Ȼл߷Ӿ۱ϩPAMΪһµˮõʡǡľءʵڵȻл߷ӣͨѧƳɡȻл߷Ӿ޶ȫص㣬һлӣڴӦڹҵƷƲƷࡣ 
        ѧ򹤳شͻƵȡãѧ߿ʼ\ˮ۱ϩPAMѸٷչоˮ۱ϩPAM˾۱ϩPAMо 
        1.2ˮ۱ϩPAMķչ 
        оͷ֣һЩˮĸϸϸ󣬵һֱδӣΪϸһַʮϸΪһּ򵥡õƷ뼼ͼƷеõ㷺Ӧá 
        ˮ۱ϩPAMһˮľܵĸ߷лҪǵסճǡάغͺȡԴҲȻл߷Ӿ۱ϩPAMȻл߷Ӿ۱ϩPAMһŵ㡣ͬʱˮ۱ϩPAMоᴿԽ뵽\ɸѡľ֣Խϵ͵ijɱøЧľ۱ϩPAMооΧѳԽ˴ͳȻл߷Ӿ۱ϩPAMо롣 
        зھ۱ϩPAMˮΪ۱ϩPAMľ۱ϩPAMButter fieldӻɸѡõ1976꣬Nakamura J.˴ùϸ߾ĸȾУɸѡ19־ˮԽù(Aspergillussouae)AJ7002ľ۱ϩPAMЧ1985꣬Takagi H.оù(Paecilomycessp.-1)ˮľ۱ϩPAMPF101PF101Կݲݸ˾󳦸˾ơƽĸѪϸࡢάطۡ̿õЧ1986꣬Kuraneúƽ(Rhodococcuserythropolis)Ƴɹˮ۱ϩPAMNOC-1Դ󳦸˾ĸཬˮˮúˮ̼ˮࡢֽˮȾмõɫЧĿǰֵĵˮ۱ϩPAM 
        ҲƳһЩԾ꣬ˮһƳҺԿˮˮ帻Ӫ͵ 
        1.3ˮ۱ϩPAM 
        ֵľԵˮ17ϣùϸ߾ͽĸ ˮ۱ϩPAM࣬дԵΪNakamura J.ýù(Aspergillussouae)ľ۱ϩPAMAJ7002 Takagi H.ù((Paecilomycessp.-1)ˮľ۱ϩPAMPF101 Kuraneúƽ(Rhodococcuserythropolis)Ƴɹˮ۱ϩPAMNOC-1 
        1.4ˮ۱ϩPAM 
        ˮ۱ϩPAM͵߷ɡˣл߷Ӿ۱ϩPAMƣʡöл߷Ӿ۱ϩPAMƣԼԡ 
        ˮ۱ϩPAMһȻ޶л߷ӻԵʺͻԶʣˮ۱ϩPAMɷֽԵĶʣҶԻ޶޺ˮ۱ϩPAMнϸߵȶԣC-62ľ۱ϩPAM10Ӻ88%ˮ۱ϩPAMԶԼϳɸ߷Ӿ۱ϩPAMĿɫʶ 
        2ˮ۱ϩPAMоӦ״ 
        2.1۱ϩPAMˮ۱ϩPAMȡʹ 
       1۱ϩPAM ۱ϩPAMͬˮͬҪӰΪCԴNԴ¶ȣʼpHֵͨٶȵȡֵעǣijһض֣۱ϩPAMͬϸ ɷֶнϴӰ졣ǻӪḻھ۱ϩPAMøߵǵʹ۱ϩPAMIJ͡ ʵ¶һΪ2530档¶̫ͻʹԽͣٶ¶̫߻ʹľ۱ϩPAMԽϵ͡
    ʼֵΪ6.08.5ڲͬľ֣pHֵвͬʼpHֵ߻;ھ۱ϩPAMIJ ڴͨһһҲֹܷɽϴʹҡʱɲ100200r/minкڿʵͨ 
       2ˮ۱ϩPAMȡʹ ˮ۱ϩPAMȡù˻ĵķȥ壬ӷҺɳɷݼʵʲͬҴͪҵȳáڽṹϸӵľ۱ϩPAMȡҪᡢлܼܽ⡢ԵõƷƷˮ򻺳Һͨӽɫ׵ȷʹ䴿òƷ 
        PF101Ϊ侫ƹΪȡһҺ߽߼2ҴȻķ룬ȡˮ󶳸ɼõƷƷˮȥʣ֬6B(Sepharose 6B)0.04mol/L״ (Tris HCl)Һ(pH=6.7)ƽ⣬ȻͨķҺ״ ỺҺϴ״ (Tris-maleate)Һ(pH=6.5)ϴѣϴҺ2Ҵ95%Ҵϴӡɣ͵õᴿijƷPF101 
        2.2 ۱ϩPAMĻ 
        ˮ۱ϩPAMIJܻƵģĿǰΪֹѷʮλSaccharomycescerevisiaeȾɫϾade138ĦFOL1ԱԵĹܡִĿ¡ԽFOL1£ȾɫУƺתϸڣʹԵϸܣʹϸܷı䡣 
        2.3 ˮ۱ϩPAMûӰ 
       1һΪ۱ϩPAMӼͬʱ˶ϵķӣڿˡмãӶʹγ״ṹͨΪϳɵĸ߷Ӿ۱ϩPAMֻͨãˮ۱ϩPAMϳɵĸ߷Ӿ۱ϩPAMûһµġֻΪϿɡ
       2кͻ һΪӵıһиɣһɵ״Ӿ۱ϩPAMˮ￿ֽʱкͽϵIJֵɣ  ʹ٣Ӷʹ䷢ײۣҺмӻpHֵӰЧ
       3ѧӦ һΪijЩԻ뱻ӦŷӦۼɽϴķӶͨӽиԴʹӻɥʧijЩԻţԷ仯áijЩѧָ۱ϩPAMĻҪڻԻţԻž˾۱ϩPAMĻԡ 
       4ɨû һΪˮ۱ϩPAMͶһγСʱ£ѸɨˮһЩӶÿɿһֻеãʵ֤۱ϩPAMԭˮ庬ȡ 
        ۱ϩPAMķӽṹ״ŶԾ۱ϩPAMĻԶӰ졣һԽԽߣԷԸߣӴ֧Խ࣬Խ۱ϩPAMڣϸˮǿľ۱ϩPAMҲԽߡˮнӵıṹɶЧҲӰ졣һЩָˮӣرCa2+Mg2+ĴЧͽ渺ɣٽšγɡ⣬ŨCa2+Ĵڻܱ۱ϩPAMܽøá 
        2.4 ˮ۱ϩPAMĹҵӦ 
        ˮ۱ϩPAMΧ㡢Ըߣַţ಻ǿȡpHֵ¶ȵӰ죬˿Թ㷺Ӧˮ͹ҵˮС ˮˮBODߣѴúϳɸ߷Ӿ۱ϩPAMЧãNOC-1ˮЧܺã10minҺӽ͸TDC8200mg/LΪ2980mg/LODֵ157Ϊ85 ˮɫ? īˮֽҺ۷ˮΪˮɫˮмNOC-1йҺ룬ʹˮɫЧԡ? Գʴѣ紦ʲݷˮʱͻ࣬NOC-1SVIܿ290½50ָͣ˻ij ﹤ҵ? ˮ﹤ҵˮмC-62ľ۱ϩPAMǶȥʿɴ96%մɳˮ? ÷ˮҪˮҩˮ֣NOC-15minˮǶȥʷֱΪ96.65%97.9%ɵõ͸Һ 
        2.5 ҹˮ۱ϩPAMо״
        ҹˮ۱ϩPAM𲽽ֻ̨˵·ڷˮķˮз뵽C-62ľ۱ϩPAMǵƱMF3MF6MF8HF24۱ϩPAMűPalcaligenes 8724ľ۱ϩPAMƷֶ٣ʵֹҵӦǰкܶоҪ 
        3 ˮ۱ϩPAMķչ 
        ۺϷˮ۱ϩPAMоӦãΪˮ۱ϩPAMоҪƣһˮ۱ϩPAMҵӦõоƹ㣬ˮɱԵʮҪNOC-1УΪNԴĽĸ۱ȽϹռNOC-1ɱ80%ƳöˮˮţѪȡĸļ۸½2/3[14]⣬Щ۱ϩPAMȻл˹ɵĸ߷ΪCԴCorynebacteriumhydrocaboclastusú۱ϩPAMNocardiaerythropolisڽе̪ͬʱ[16]\ķٷչǶˮʶͿҲԽԽ硣͸ǴݲͬķˮˮƾԵĸЧˮ۱ϩPAMЧɴ󽵵;۱ϩPAMͶӶʹɱϵͳ˾ӸߵȺ«ȡͷżص۱ϩPAMˮЧdzáҲѧͼͨøصȴٽˮʼص۱ϩPAM(PAM)ϣʵлϣڱ֤ˮˮʵǰ£ϵͳͷˮϵͳͣʱ䣬Ӷˮ۱ϩPAMչ۱ϩPAMо롣
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